This is usually expressed as the Km (Michaelis constant) of the enzyme, an inverse measure of affinity. For practical purposes, Km is the concentration of.
I thought if the Km-value is low, substrate affinity is high and in the opposite direction. The true reciprocal measure of enzyme substrate affinity is the Ks value.
Structural Biochemistry/Enzyme/Michaelis and Menten Equation An equation with a high Km indicates that the enzyme does not bind as efficiently with the substrate, When Kcat/ Km, it gives us a measure of enzyme efficiency with a unit of.
These constants are important to know, both to understand enzyme activity The maximal velocity, or Vmax, is the rate of the reaction under these conditions.
Km is characteristic of an enzyme and a particular substrate and is a measure of enzyme affinity for that substrate. A low Km value of an enzyme.
One needs measuring tools in order to find out how enzymes work and how they are controlled. The Michealis-Menton contant is one such tool. It allows you to.